CHICAGO: In-vivo imaging has the potential to obtain quantitative information at a single-cell resolution. When this imaging technique is carried out non-invasively on fully functioning and intact organisms, time lapse microscopy enables developmental processes to be studied over time.
The speed, resolution and penetration of fluorescence microscopy techniques have become increasingly powerful, although only when applied to thin and transparent samples. It is particularly difficult to achieve single cell resolution when examining whole embryos, which are usually a few millimeters in size…